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Objective To explore the application value of interferon gamma release assay (IGRAs)in the clinical detection of tuberculosis infected T lymphocytes.Methods Used IGRAs method to detect the 11 968 outpatients and hospitalized pa-tients from 2013 to 2016 with tuberculosis screening.According to the distribution department analysis,also of positive case detection according to age and gender were analysis and comparison and analysis on the uncertainty of results,different methods were compared.Results Among the 11 968 cases,2 048 cases were positive,the positive rate was 17.11%,and the uncertain result was 107 cases,which accounted for 0.89% of the total number.The positive rates from 2013 to 2016 were 19.65%,21.35%,15.82% and 13.56%,respectively.In the detection and screening of pulmonary and pulmonary tuberculo-sis,the positive rates of the department of respiration,the digestive department,the oncology department,the department of neurology and the department of gynecology were 22.07%,20.27%,23.38%,12.84% and 11.86%,respectively.In the positive screening,men accounted for 62.11%,women accounted for 37.89%,men were significantly higher than women.By age group,was less than or equal to 15,16~25,26~45,46~65,was more than orequal to 66 years old,positive rate were 1.96%,18.51%,16.54%,21.25% and 25.73%,respectively.Analysis of uncertain outcome data,department of respira-tion,rheumatism,department of hematology,accounted for 1.99% and 2.35%,respectively.Compared with other laboratory methods,the IGRAs method had obvious advantages.Conclusion Tuberculosis occurs in various body organs,there were differences in gender and age of Mycobacterium tuberculosis infection.IGRAs is a sensitive and specific method for rapid de-tection of Mycobacterium tuberculosis infection,although it can not be used as a diagnostic indicator,but in patients with suspected tuberculosis IGRAs has a larger clinical application value for the further diagnosis of disease.
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Objective To compare the autoantibody detection and enzyme linked immunosorbent assay of protein chip in the diagnosis of rheumatoid arthritis (RA).Methods 81 cases of RA were given autoantibodies protein chip and enzyme linked immunosorbent assay(ELISA),the project included rheumatoid factor(RF)and anti cyclic citrullinated peptide antibody(Anti -CCP),anti nuclear factor(APF),anti keratin antibody(AKA)and anti-nuclear antibody(ANA),recorded the test results,time and cost,the income data were statistically analyzed.Results Patients with RA autoantibody protein chip for detection of CCP,AKA,RF,APF,ANA positive rates were 93.83%, 92.59%,90.12%,95.06%,92.59%,ELISA for the detection of CCP,AKA,RF,APF and ANA positive rates were 95.06%,91.36%,92.59%,91.36%,93.83%,two detection methods had no significant differences(χ2 =1.908, 1.345,1.764,2.246,1.102,all P >0.05);autoantibody detection protein chip required time and cost were respec-tively (1.33 ±0.19)h,(151.24 ±29.83)yuan,which were significantly less than the detection of ELISA (6.81 ± 1.24)h,(899.67 ±121.35)yuan,the differences were statistically significant(t =7.989,8.235,all P <0.05 ). Conclusion The detection of antibody protein chip for RA diagnosis with high accuracy,short time,less cost,help the doctor to diagnose the disease quickly obtain test results.
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Objective To study the possibility of direct identification of pathogens from positive blood cul-tures by methods of separation gel tube -centrifugation.Methods 216 cases of positive blood culture were collected from 2015.7 to 2015.12.The bacterias were purified from blood culture bottle by separation gel tube.After washing 2 times,identified by MALDI -TOF MS.At the same time,traditional culture,smears and identification were done. Compared the results of identification by two methods.Results 216 cases of positive blood culture were single bacte-rial infection.By Gram stain,89 strains were Gram positive,119 strains were Gram negative and 8 strains were fungal spores.190 cases of positive blood culture were identified by MALDI -TOF MS,it concluded 67 Gram positive strains,111 Gram negative strains,4 anaerobe strains and 8 fungus.Compared with traditional culture,the coincidence rate reached up to 87.9%,Gram positive strains 78.8%,Gram negative strains 93.2%,anaerobe strains 100.0%and fungus 100.0%.Conclusion It takes less than 30 minutes purified from blood culture bottle by separation gel tube.And the time of identification is shorter than traditional culture.This method is good for clinical diagnosis and treatment.
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Objective To evaluate the correlation of the ion selectivity electrode assay and arsenazo Ⅲ assay for serum calcium determination and compare the differences between two methods.Methods 142 serum samples were collected from patients and detected by ion selectivity electrode assay and arsenazo Ⅲ assay,the differences of two methods were compared,person correlation and Bland -Altman was used to discuss the correlation.Results The serum calcium concentration detected by ion selectivity electrode assay was (2.23 ±0.10)mmol/L and detected by arsenazo Ⅲ assay was (2.24 ±0.12)mmol/L,the difference between the two methods was not statistically significant (t =-1.09,P >0.05).The person correlation analysis demonstrated that the two methods had a positive correlation (r =0.393,P <0.01).The Bland -Altman consistency analysis showed that ion selectivity electrode assay was agreed with arsenazo Ⅲ assay.Conclusion The two methods for serum calcium concentration determination,the ion selectivity electrode assay was coincidence with the arsenazo Ⅲ assay.
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Urinary excretion of albumin indicates kidney damage and is recognized as a risk factor for progression of kidney disease and cardiovascular disease,resulting a widespread clinical utilization of urine albumin measurement.Considerable inter-method difference has been reported for urine albumin,there are no available reference materials and no reference measurement procedures for urine albumin.This review discusses the clinical utilization of urine albumin in recent years,the principles of existing measurement systems and difference of results among systems,and the current status of work about reference materials and reference measurement procedures.
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Application of standard materials in clinical laboratory medicine plays an important role in assuring result concordance between different reagents, methods and clinical laboratories. This article provides an overview of the correct classification, selection and application of standard materials for clinical laboratory medicine.
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The test panels are combined for the clinical application according to the principle of the evidence-based medicine, which involves the strict evaluation for the technical performance, diagnostic performance, the result of patients and the economic properties. The guideline for the clinical application of the test panels is established in light of different diagnostic objectives and diseases, providing the best diagnostic service for the physicians and benefiting the patients the most.